Frequently Asked Questions – Fixed  

Can I calibrate the Ovation pipette myself?

How often should I verify calibration?

I can’t reach the bottom of my taller sample tubes. What do I do?

What is the difference between a one stroke and a two stroke pipette?

Are “custom” fixed volume models available?

Can I calibrate the Ovation pipette myself? return to faq

Yes. An Allen key is provided for calibration adjustment. To raise the volume delivery, insert the Allen key into the recessed set screw on the top surface near the hook and turn the Allen key clockwise. To decrease the volume delivery, turn the Allen key counterclockwise.

How often should I verify calibration? return to faq

Reputable pipetting reference documents recommend that calibration verification be performed at least every six months. In addition, VistaLab Technologies suggests that calibration verification be performed for any of the following reasons:

• Maintenance such as seal replacement has been performed
• Quality control sampling shows a change in results
• Current laboratory protocols and practices require more frequent verification

I can’t reach the bottom of my taller sample tubes. What do I do? return to faq

An extended nozzle that increases the pipette’s reach by 1” is available for models dispensing 200µL or less. This allows the tip to reach the bottom of even 50mL centrifuge tubes. The larger volume capacity tips used on Ovation pipettes for dispensing 201 to 1000µL easily reach the bottom of taller sample tubes without a nozzle extension. Order VistaLab Cat. #9057-1012 .

What is the difference between a one stroke and a two stroke pipette? return to faq

To aspirate and dispense fluid with a one stroke pipette, the plunger is fully depressed and then released during either operation. Ovation pipettes with the one stroke feature are calibrated “to deliver” (TD). With two stroke pipettes, the plunger has a “stop” approximately midway to the bottom of its stroke. To aspirate fluid, the plunger is depressed to the stop and then released. To dispense, the plunger is depressed to the stop, then after one second it is fully depressed to initiate “overblow”, forcing any remaining liquid out of the tip. Ovation pipettes with the two stroke feature are calibrated “to contain” (TC). In addition, two stroke models allow “reverse pipetting”, which can offer increased accuracy and precision with viscous fluids or those with varying densities, or micro volumes. To “reverse pipette”, depress the plunger fully to the bottom and fully release the plunger to aspirate. To dispense, depress the plunger ONLY to the first stop. Discard any remaining liquid in the tip.

Are “custom” fixed volume models available? return to faq

Yes. VistaLab Technologies can custom manufacture Ovation fixed volume pipettes to aspirate and dispense any designated volume between 1µL and 1000µL. Contact VistaLab Technical Services at (888) 652-6520 with your specific requirements.