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Frequently Asked Questions – General

Do other manufacturer’s tips fit the Ovation pipette?


Why is the manufacturing quality of a tip so important?

Can I calibrate the Ovation pipette myself?

How often should I verify calibration?


At what volume should I dispense when verifying calibration or changing calibration for a specific liquid?

What if I have to send in my Ovation for calibration, maintenance or service?

What is the cost of calibration, maintenance or other services?

What is the warranty period for Ovation Pipettes?
 

Is calibration covered under warranty? 

Should I pre-wet tips? 


Should I rinse out the tip when I am dispensing?

Can the Ovation Pipette be autoclaved? 

How can I decontaminate the pipette? 

What is the purpose of the liquid blocking nozzle?
 

How often do I change the filter in the nozzles?


My pipette is not aspirating. What could be the problem?

How often do I need to replace seals? 


How do I know it is time to replace seals? 

What tips are available? 

Do other manufacturer’s tips fit the Ovation pipette?  return to faq
 
Some other manufacturer’s tips do fit Ovation pipettes, however we can not assure that our stated performance specifications can be met when other tips are used. All performance claims in our literature and on this web site are based on using the Ovation pipette with Ovation tips as a system. Additionally, the tip acquisition and tip ejection functions may not perform properly when other tips are used. See the Ovation Tip section of this web site for more information on this topic.

Why is the manufacturing quality of a tip so important?  return to faq  

Tip quality can affect pipetting results in a number of ways: 

  • Hydrophobic qualities - if a tip’s hydrophobic properties are diminished, it could retain liquid inside, or even retain a drop of measured liquid on the outer surface.
  • Poor quality tips may have burrs or plastic flash which do not allow the smooth release of all liquid within the tips – some of the measured volume may be trapped at the end of the tip.
  • Non-concentric tip openings to the end of the tip affect the dispensing of the contents. Some liquid may adhere due to changes in surface properties.
  • Tip straightness affects the dynamics of the air displacement. 

Can I calibrate the Ovation pipette myself?    return to faq  

Yes. There are separate procedures depending upon the model. 

  • For Ovation multichannel and adjustable volume models, software for in-lab calibration is internal to the pipette. Once you have determined the actual volume that the pipette is delivering, this value can be directly entered into the calibration software. That’s all it takes. A known calibration factor for any liquid can also be entered at any time.
  • For Ovation fixed volume models, an Allen key is provided for calibration adjustment. To raise the volume delivery, insert the Allen key into the recessed set screw on the top surface near the hook and turn the Allen key clockwise. To decrease the volume delivery, turn the Allen key counterclockwise.

How often should I verify calibration?    return to faq  

Reputable pipetting reference documents recommend that calibration verification be performed at least every six months. In addition, VistaLab Technologies suggests that calibration verification be performed for any of the following reasons:

  • Maintenance such as seal replacement has been performed
  • Quality control sampling shows a change in results
  • Current laboratory protocols and practices require more frequent verification  

At what volume should I dispense when verifying calibration or changing calibration for a specific liquid?    return to faq

  • For pipetting performance across the entire range, calibration verification should be based on the volume delivery at the pipette’s maximum volume.
  • For optimum performance with a specific liquid at a set volume, use that volume setting. If the specific liquid is dispensed at multiple volumes, then use the pipette’s maximum volume. 

What if I have to send in my Ovation for calibration, maintenance or service?    return to faq  

Complete information about our calibration and repair services is available in the Support area of this web site under “Service”. Routine turnaround time for these services is two business days.

What is the cost of calibration, maintenance or other services?    return to faq  

Complete pricing information about our calibration and repair services is available in the Support area of this web site under “Service”. Costs are based on the specific services that need to be performed.   

What is the warranty period for Ovation Pipettes?    return to faq  

VistaLab Technologies, Inc. warrants the Ovation BioNatural Pipette against defects in materials and workmanship for one year from the date of purchase. To register your pipette and activate the warranty, go to the Support area of this web site, and click on “Pipette Registration”. Complete and submit the on-line form provided.

Is calibration covered under warranty?    return to faq  

No. Each Ovation pipette is sent to you with a calibration certificate that is generated at the time of manufacturing. This assures you that when the pipette leaves our factory, it is calibrated by trained technicians to the performance specifications using the reference (gravimetric) method. If the environment, techniques or methodologies used in your laboratory differ from those used in the reference (gravimetric) method, a simple in-lab procedure using calibration software internal to Ovation pipettes with the adjustable volume feature, or an Allen key for fixed volume models, may be followed to make any necessary adjustments.     

Should I pre-wet tips?    return to faq  

Reference documents (ISO/DIS 8655) recommend that prior to use, each pipette tip should be pre-wet in the sample to be used. This greatly increases the accuracy and precision of your work.  

Should I rinse out the tip when I am dispensing?    return to faq  

No. Pipettes are calibrated to deliver (TD) or to contain (TC). Rinsing out will change the amount of calibrated liquid being dispensed. 

Can the Ovation Pipette be autoclaved?    return to faq  

No. However, the pipette can be decontaminated by wiping down the outside surface (except for the LCD screen) with a 70% aqueous solution of ethanol or isopropanol, or use a 10% bleach solution followed by water. ONLY WIPE THE LCD SCREEN WITH WATER.  

How can I decontaminate the pipette?    return to faq  

See above question on autoclaving  

What is the purpose of the liquid blocking nozzle?    return to faq  

The liquid blocking nozzle contains an internal aerosol/liquid barrier filter to prevent liquid from being aspirated into the pipette. It also offers some protection to internal parts from routine exposure to hazardous liquids and vapors. If this filter becomes wet, the pipette will not aspirate fluid until a new nozzle is installed.  

How often do I change the filter in the nozzles?   return to faq

Nozzles should be changed if any liquid has been accidentally aspirated into the nozzles (such as when the volume setting of the pipette exceeds the capacity of the installed tips).   In addition, filters should be replaced on an as-needed basis depending on the solutions that are routinely pipetted or if the nozzle filters are contaminated.  

My pipette is not aspirating. What could be the problem?    return to faq

Verify that the nozzle and/or extender is firmly tightened. Then ruleout that the liquid blocking nozzle has not become wet by changing the nozzle. If it still is not aspirating, contact VistaLab Technical Services.  

How often do I need to replace seals?    return to faq  

The seals should not require changing unless the pipette has been used with chemicals that are harmful to or negatively affect rubber. Generally, seals do not need to be changed more than one time every 2-3 years.   

How do I know it is time to replace seals?    return to faq

  • Precision and accuracy are not within specifications, and technique and environment are not a factor
  • The pipette’s plunger is not moving freely
  • The pipette leaks and this condition is not related to tips

What tips are available?    return to faq  

Micro, small and large volume tip sizes are available specifically for the Ovation pipette to ensure proper fit and easy tip acquisition and ejection. They are precision molded to the strictest dimensional standards for consistent tip-to-tip and lot-to-lot performance. A variety of packaging formats are available as well as specific tips that meet the certification requirements needed in many applications. See the Ovation Tips and Accessories section of this web site for more information.